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Series GSE29714 Query DataSets for GSE29714
Status Public on May 17, 2012
Title Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3' UTRs and Near Stop Codons
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Here, we use a novel technique for locating regions of N6-adenosine methylation (m6A) throughout the transcriptome and present a profile of m6A sites in the mouse brain. Our use of methylated RNA immunoprecipitation combined with RNA-seq (MeRIP-Seq) identifies thousands of RNAs which contain m6A sites. In addition, we find that regions of m6A formation are particularly enriched near stop codons, which might provide clues into the potential funciton of this highly prevalent RNA modificaiton.
Overall design Examination of m6A sites in murine brain RNA and human embryonic kidney cells.
Contributor(s) Meyer KD, Saletore YV, Elemento O, Zumbo P, Mason CE, Jaffrey SR
Citation(s) 22608085
Submission date Jun 03, 2011
Last update date May 15, 2019
Contact name Christopher E Mason
Organization name Weill Cornell Medical College
Department Physiology and Biophysics
Lab Christopher Mason Lab
Street address 1305 York Ave
City New York
ZIP/Postal code 10065
Country USA
Platforms (3)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (11)
GSM854223 C57BL/6 Brain_Sample 1 MeRIP - SYSY
GSM854224 C57BL/6 Brain_Sample 1 Non-IP Control
GSM854225 C57BL/6 Brain_Sample 2_Non-IP Control
BioProject PRJNA141085
SRA SRP007335

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Supplementary file Size Download File type/resource
GSE29714_RAW.tar 1.4 Gb (http)(custom) TAR (of BW)
GSE29714_m6a_vs_ctrl.txt.gz 375.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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