|
| Status |
Public on Dec 20, 2012 |
| Title |
toll10b 2-4h replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
Toll10b mutant
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genetic background: embryos were collected from Toll10b/+ females obtained from a balanced stock (Toll10b/TM3, Sb, Ser and Toll10b/OR60)
tissue: whole-embryo
time point: 02 to 04h AEL
|
| Growth protocol |
D. melanogaster embryos were collected and aged on yeasted apple juice plates at 25 °C and 60 % humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA-seq: polyA mRNA was purified from total RNA in two consecutive rounds of oligo(dT) selection. After RNA fragmentation and reverse transcription sequencing libraries were prepared following Illumina's instructions (see ChIP-seq)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
toll10b_02to04h_1.bam; genome build: dm3
Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
|
| |
|
| Submission date |
Dec 08, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Julia Zeitlinger |
| E-mail(s) |
jbz@stowers.org
|
| Organization name |
Stowers Institute for Medical Research
|
| Lab |
Zeitlinger Lab
|
| Street address |
1000 E 50th St
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
|
| Platform ID |
GPL11203 |
| Series (2) |
| GSE34281 |
Poised RNA Polymerase II changes over developmental time and prepares genes for future expression (RNA-seq data) |
| GSE34304 |
Poised RNA Polymerase II changes over developmental time and prepares genes for future expression |
|
| Relations |
| Reanalyzed by |
GSM3276070 |
| SRA |
SRX110699 |
| BioSample |
SAMN00764141 |
