|
Status |
Public on Dec 20, 2012 |
Title |
muscle 8-10h replicate 2 |
Sample type |
SRA |
|
|
Source name |
muscle FAC sorted from dissociated embryos
|
Organism |
Drosophila melanogaster |
Characteristics |
genetic background: P{w+mC=UAS-mCD8::GFP.L}LL6, P{w+mC=Dmef2-GAL4} tissue: muscle time point: 08 to 10h AEL
|
Growth protocol |
D. melanogaster embryos were collected and aged on yeasted apple juice plates at 25 °C and 60 % humidity.
|
Extracted molecule |
total RNA |
Extraction protocol |
mRNA-seq: polyA mRNA was purified from total RNA in two consecutive rounds of oligo(dT) selection. After RNA fragmentation and reverse transcription sequencing libraries were prepared following Illumina's instructions (see ChIP-seq)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer IIx |
|
|
Data processing |
mef2_08to10h_2.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
|
|
|
Submission date |
Dec 08, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Julia Zeitlinger |
E-mail(s) |
jbz@stowers.org
|
Organization name |
Stowers Institute for Medical Research
|
Lab |
Zeitlinger Lab
|
Street address |
1000 E 50th St
|
City |
Kansas City |
State/province |
MO |
ZIP/Postal code |
64110 |
Country |
USA |
|
|
Platform ID |
GPL11203 |
Series (2) |
GSE34281 |
Poised RNA Polymerase II changes over developmental time and prepares genes for future expression (RNA-seq data) |
GSE34304 |
Poised RNA Polymerase II changes over developmental time and prepares genes for future expression |
|
Relations |
Reanalyzed by |
GSM3276065 |
SRA |
SRX110694 |
BioSample |
SAMN00764136 |