|
| Status |
Public on Nov 01, 2012 |
| Title |
PtAIL1 RNAi line T72-1 |
| Sample type |
RNA |
| |
|
| Source name |
Stem bases of PtAIL1 RNAi line after 72h of rooting
|
| Organism |
Populus tremula x Populus tremuloides |
| Characteristics |
clone: T89
tissue: 2mm of the stem base from the base of excised shoots
genotype/variation: PtAIL1 RNAi line
time: 72 hr
|
| Biomaterial provider |
Umea Plant Science Center
|
| Growth protocol |
Cuttings of hybrid aspen (Populus tremula x tremuloides) clone T89 (wild type) and the transgenic lines were grown in half-strength Murashige/Skoog medium (½ MS) under sterile conditions for approximately 4 weeks and then used for In vitro rooting experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy micro kit (QIAGEN). We added a first clean-up step with QIAshredder Mini Spin Columns (QIAGEN) and an on-column DNA digestion step with DNAse I (Qiagen). RNA quality
was verified by Experion StdSens capillary gels (Bio-Rad). Total RNA preparations were amplified using the SMARTer PCR cDNA Synthesis Kit (Clontech) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company
|
| Scan protocol |
Scanning by NimbleGen Company
|
| Data processing |
Expression values reflect across-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA). For information on RMA see Biostatistics 2003 Apr; 4(2): 249-264
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL13485 |
| Series (1) |
| GSE34162 |
Altered PtAIL1 expression is accompanied by significant transcriptome changes during adventitious rooting induction |
|
