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| Status |
Public on Nov 01, 2012 |
| Title |
Altered PtAIL1 expression is accompanied by significant transcriptome changes during adventitious rooting induction |
| Platform organism |
Populus trichocarpa |
| Sample organism |
Populus tremula x Populus tremuloides |
| Experiment type |
Expression profiling by array
|
| Summary |
Oligoarray analysis was used to identify genes differentially expressed in 2 mm sections of the basal stem region of wild type T89, PtAIL1 over-expressing or PtAIL1 RNAi lines of P. tremula x Populus tremuloides 24h and 72h after adventitious rooting induction.
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| Overall design |
The Populus trichocarpa custom-exon expression array (12x135K) manufactured by Roche NimbleGen Systems Limited (Madison, WI) (http://www.nimblegen.com/products/exp/index.html) contained three independent, nonidentical, 60-mer probes per gene model coding sequence. Included in the oligoarray were 43,929 annotated gene models (Populus trichocarpa genome v2 ; Phytozome 5.0), 5,859 random 60-mer control probes and labelling controls. We performed 27 hybridizations from wildtype, PtAIL1 over-expressing or PtAIL1 RNAi lines of P. tremula x Populus tremuloides at time 0, 24h and 72h after adventitious rooting induction. For each timepoint we performed three biological replicates.
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| Contributor(s) |
Rigal A, Kohler A, Karlberg A, Tisserant E, Legué V, Bhalerao R, Martin F |
| Citation(s) |
23077242 |
| |
| Submission date |
Dec 05, 2011 |
| Last update date |
Jan 08, 2013 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
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| Platforms (1) |
| GPL13485 |
Populus trichocarpa 12-plex whole genome expression array |
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| Samples (27)
|
| GSM842851 |
Populus tremula x Populus tremuloides T89 wild type T0-1 |
| GSM842852 |
Populus tremula x Populus tremuloides T89 wild type T0-2 |
| GSM842853 |
Populus tremula x Populus tremuloides T89 wild type T0-3 |
| GSM842854 |
PtAIL1 over-expressing line T0-1 |
| GSM842857 |
PtAIL1 over-expressing line T0-2 |
| GSM842858 |
PtAIL1 over-expressing line T0-3 |
| GSM842859 |
PtAIL1 RNAi line T0-1 |
| GSM842860 |
PtAIL1 RNAi line T0-2 |
| GSM842861 |
PtAIL1 RNAi line T0-3 |
| GSM843207 |
Populus tremula x Populus tremuloides T89 wild type T24-1 |
| GSM843208 |
Populus tremula x Populus tremuloides T89 wild type T24-2 |
| GSM843209 |
Populus tremula x Populus tremuloides T89 wild type T24-3 |
| GSM843320 |
PtAIL1 over-expressing line T24-1 |
| GSM843321 |
PtAIL1 over-expressing line T24-2 |
| GSM843322 |
PtAIL1 over-expressing line T24-3 |
| GSM843323 |
PtAIL1 RNAi line T24-1 |
| GSM843324 |
PtAIL1 RNAi line T24-2 |
| GSM843325 |
PtAIL1 RNAi line T24-3 |
| GSM843326 |
Populus tremula x Populus tremuloides T89 wild type T72-1 |
| GSM843327 |
Populus tremula x Populus tremuloides T89 wild type T72-2 |
| GSM843328 |
Populus tremula x Populus tremuloides T89 wild type T72-3 |
| GSM843329 |
PtAIL1 over-expressing line T72-1 |
| GSM843330 |
PtAIL1 over-expressing line T72-2 |
| GSM843331 |
PtAIL1 over-expressing line T72-3 |
| GSM843332 |
PtAIL1 RNAi line T72-1 |
| GSM843333 |
PtAIL1 RNAi line T72-2 |
| GSM843334 |
PtAIL1 RNAi line T72-3 |
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| Relations |
| BioProject |
PRJNA150045 |
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