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Sample GSM840949 Query DataSets for GSM840949
Status Public on Nov 30, 2011
Title HepG2_Stearic acid_rep2
Sample type RNA
Source name HepG2, Stearic acid, 7 days
Organism Homo sapiens
Characteristics cell line: HepG2
incubated fatty acid: Stearic acid
Treatment protocol Incubation for seven days with 100 µM fatty acid (oleic, elaidic or stearic acid)
Extracted molecule total RNA
Extraction protocol Total RNA Purification Kit (NORGEN BIOTEK CORPORATION) Distribubed by Phalanx Biotech
Label Cy5
Label protocol RNA was converted to double-stranded cDNA and amplified using an in vitro transcription reaction that included aminoallyl-UTP, and the aRNA product was conjugated with Cy5T NHS ester
Hybridization protocol Fragmented aRNA was hybridized at 42° C overnight using the HybBag mixing system with 1× OneArray Hybridization Buffer (Phalanx Biotech), 0.01 mg/mL sheared salmon sperm DNA (Promega) and 0.025 mg/mL labeled target. After hybridization, the arrays were washed according to the OneArray protocol
Scan protocol Raw intensity signals from each microarray were captured using a Molecular Dynamics™ Axon 4100A scanner, measured using GenePixPro™ Software, and stored in GPR format
Description SB
raw data file technical rep1: H027-0607002924.gpr
raw data file technical rep2: H028-0607002925.gpr
Data processing The data from all microarrays in each experimental set were passed to Rosetta Resolver (Rosetta Biosoftware) for analysis. Testing was performed by combining the technical replicates and performing statistical analyses using Rosetta Resolver's proprietary modeling techniques
Submission date Nov 30, 2011
Last update date Nov 30, 2011
Contact name Toke Krogager Hansen
Organization name Aarhus University
Department Molecular Biology
Lab Proteome analysis
Street address gustav wieds vej 10c
City Aarhus
ZIP/Postal code 8000
Country Denmark
Platform ID GPL6254
Series (1)
GSE34045 Identification of a biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Data table header descriptions
VALUE Normalized signal Intensity calculated by Rosetta Resolver

Data table
PH_hs_0000002 180.351852
PH_hs_0000003 12.0459766
PH_hs_0000004 112.055061
PH_hs_0000005 6.99628448
PH_hs_0000006 612.974548
PH_hs_0000007 343.821777
PH_hs_0000008 19817.4023
PH_hs_0000009 52.4301529
PH_hs_0000010 2198.52612
PH_hs_0000011 102.722862
PH_hs_0000012 342.220642
PH_hs_0000013 517.368164
PH_hs_0000014 535.406616
PH_hs_0000015 1829.13342
PH_hs_0000016 1569.13342
PH_hs_0000017 169.496735
PH_hs_0000018 20.5136566
PH_hs_0000019 8.49439812
PH_hs_0000020 4052.65625
PH_hs_0000021 126.864441

Total number of rows: 30968

Table truncated, full table size 754 Kbytes.

Supplementary file Size Download File type/resource
GSM840949_H027-0607002924.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM840949_H028-0607002925.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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