All the CP-r cells were maintained in the presence of cisplatin, but cisplatin was removed from the medium 3 days prior to preparation of RNA. See Gillet et al. (Manuscript in in submision)
Growth protocol
Clinical samples were fresh frozen. Cell lines were grown either as monolayer or in 3-D (see Gillet et al. Manuscript in submission)
Extracted molecule
total RNA
Extraction protocol
Total RNA was prepared using the Trizol method (Invitrogen, Carlsbad, CA, USA).
Label
FAM
Label protocol
cDNA was prepared using the High Capacity cDNA kit with RNAse inhibitor (Applied Biosystems, Foster City, CA) as per the manufacturer’s instructions, but no labelling was performed as TaqMan-based qRT-PCR was performed subsequently.
Hybridization protocol
n/a
Scan protocol
ABI Prism 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA)
Description
Clinical sample
Data processing
SDS files were processed in RQ manager software and raw data were then exported in XLS file
