|
| Status |
Public on Nov 28, 2011 |
| Title |
Clinical sample, B-N1 r2 133- |
| Sample type |
RNA |
| |
|
| Source name |
Normal breast tissue
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: CD133- sorted cells
|
| Treatment protocol |
All the CP-r cells were maintained in the presence of cisplatin, but cisplatin was removed from the medium 3 days prior to preparation of RNA. See Gillet et al. (Manuscript in in submision)
|
| Growth protocol |
Clinical samples were fresh frozen. Cell lines were grown either as monolayer or in 3-D (see Gillet et al. Manuscript in submission)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the Trizol method (Invitrogen, Carlsbad, CA, USA).
|
| Label |
FAM
|
| Label protocol |
cDNA was prepared using the High Capacity cDNA kit with RNAse inhibitor (Applied Biosystems, Foster City, CA) as per the manufacturer’s instructions,
but no labelling was performed as TaqMan-based qRT-PCR was performed subsequently.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
ABI Prism 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA)
|
| Description |
Clinical sample
|
| Data processing |
SDS files were processed in RQ manager software and raw data were then exported in XLS file
|
| |
|
| Submission date |
Nov 23, 2011 |
| Last update date |
Nov 28, 2011 |
| Contact name |
jean-pierre gillet |
| E-mail(s) |
jean-pierre.gillet@unamur.be
|
| Phone |
003281724275
|
| Organization name |
University of Namur
|
| Department |
URPhyM
|
| Lab |
LMCB
|
| Street address |
61 Rue de Bruxelles
|
| City |
Namur |
| ZIP/Postal code |
5000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL14924 |
| Series (1) |
| GSE30034 |
Redefining the Relevance of Established Cancer Cell Lines to the Study of Clinical Anti-Cancer Drug Resistance |
|
