|
| Status |
Public on Apr 30, 2012 |
| Title |
SSc-ILD-PH_PAH-49 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: Interstitial Lung Disease
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Venous blood was collected by simple venipuncture under aseptic conditions. All samples were processed within two hours of collection to minimize gene expression variations associated with longer sample incubation times. PBMCs were separated by Ficoll density gradient, and were immediately lysed in Trizol reagent (Invitrogen, Carlsbad, California). Additional purification was performed on RNeasy columns (Qiagen, Valencia, CA 913555, cat. no. 74104). The quality of total RNA samples was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
Total RNA was labled with the Ambion TotalPrep RNA Amplification Kit according to manufacturers suggested protocols.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Interstitial Lung Disease
|
| Data processing |
A single intensity (expression) value for each Illumina probe on the array was obtained using Illumina BeadStudio software with standard settings and no background correction. The expression values for all the probes for each sample were scaled to have median 256 (28) and then log (base 2) transformed before performing statistical analysis.
|
| |
|
| Submission date |
Nov 03, 2011 |
| Last update date |
Apr 30, 2012 |
| Contact name |
chris cheadle |
| E-mail(s) |
ccheadl1@jhmi.edu
|
| Phone |
4105505984
|
| Organization name |
JHU
|
| Department |
SOM
|
| Lab |
JHBMC Genomics Core
|
| Street address |
5200 Eastern Ave
|
| City |
baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE33463 |
Erythroid-Specific Transcriptional Changes in PBMCs from Pulmonary Hypertension Patients |
|
