|
| Status |
Public on Dec 20, 2011 |
| Title |
secondary growth lV3 |
| Sample type |
RNA |
| |
|
| Source name |
ovary
|
| Organism |
Micropterus salmoides |
| Characteristics |
tissue: ovary
Sex: female
Stage: secondary growth lV
|
| Treatment protocol |
wild largemouth bass sampled each month over 1 year, ovary sample taken for microarray analysis on reprodcutive tissue, n=4/stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA for microarray analysis was extracted from LMB ovary using RNA STAT-60 reagent (TEL-TEST Inc., Friendswood, TX, USA).
|
| Label |
Cy3
|
| Label protocol |
Agilent one color protocol for Cy3
|
| |
|
| Hybridization protocol |
Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
|
| Scan protocol |
Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software (v9.5) formed a composite of the two scans and calculated parameters for Extended Dynamic Range.
|
| Description |
1196-20
secondary growth lV sample 3
|
| Data processing |
Normalized signal intensity provided here is LOESS Normalization using JMP Genomics v4.0 software
|
| |
|
| Submission date |
Oct 12, 2011 |
| Last update date |
Dec 20, 2011 |
| Contact name |
Christopher Joseph Martyniuk |
| E-mail(s) |
CMARTYN@UFL.EDU
|
| Organization name |
UF
|
| Department |
Physiological Sciences
|
| Street address |
CEHT Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
| |
|
| Platform ID |
GPL13229 |
| Series (1) |
| GSE32930 |
Ovarian transcriptomics over a complete breeding cycle in wild Largemouth bass (Micropterus salmoides) characterizes pathways underlying oocyte maturation and atresia |
|
