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Sample GSM8149649 Query DataSets for GSM8149649
Status Public on Mar 18, 2024
Title Liver, water, db/+, rep1
Sample type SRA
 
Source name Liver
Organism Mus musculus
Characteristics tissue: Liver
Sex: Male
strain: C57/BL6
genotype: db/db
treatment: 16 weeks
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tissue samples using the TRIzolTM Reagent. 1 mL of the reagent and a stainless-steel bead were added to the tissue samples, and tissues were fully homogenized by the TissueLyser LT homogenizer.
1000 ng of total RNA was processed to mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (New England BioLabs, MA, USA) and followed by fragmentation, cDNA synthesis, and library preparation with unique dual index using NEBNext Ultra II RNA library prep kit (New England BioLabs) according to the manufacturer’s manual.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Raw sequencing data were processed using Illumina bcl2fastq conversion tool to generate FASTQ files
Sequences were aligned to GRCm39 genome to gene expression count using New Tuxedo Protocol's default settings
Gene expression level of Refseq coding genes were quantified using Ballgown tool which inspect the distribution of abundance estimates.
Assembly: GRCm39
Supplementary files format and content: gene_expression_fpkm.txt
 
Submission date Mar 15, 2024
Last update date Mar 18, 2024
Contact name sungguan hong
E-mail(s) sungguanhong@cau.ac.kr
Organization name Chung-Ang
Department Chemistry
Street address 84, Heukseok-ro, Dongjak-gu, Seoul, 104-407
City seoul
State/province none
ZIP/Postal code 06974
Country South Korea
 
Platform ID GPL24247
Series (1)
GSE261723 3M-Brazzein, a Natural Sugar Substitute, can Suppress Metabolic Disorders, NAFLD, and Depression-like Behaviors in Diabetic Mice
Relations
BioSample SAMN40474919
SRA SRX23964467

Supplementary file Size Download File type/resource
GSM8149649_Het-W-L1.txt.gz 191.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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