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| Status |
Public on Mar 18, 2024 |
| Title |
Liver, water, db/+, rep1 |
| Sample type |
SRA |
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| Source name |
Liver
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| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
Sex: Male
strain: C57/BL6
genotype: db/db
treatment: 16 weeks
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tissue samples using the TRIzolTM Reagent. 1 mL of the reagent and a stainless-steel bead were added to the tissue samples, and tissues were fully homogenized by the TissueLyser LT homogenizer.
1000 ng of total RNA was processed to mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (New England BioLabs, MA, USA) and followed by fragmentation, cDNA synthesis, and library preparation with unique dual index using NEBNext Ultra II RNA library prep kit (New England BioLabs) according to the manufacturer’s manual.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Raw sequencing data were processed using Illumina bcl2fastq conversion tool to generate FASTQ files
Sequences were aligned to GRCm39 genome to gene expression count using New Tuxedo Protocol's default settings
Gene expression level of Refseq coding genes were quantified using Ballgown tool which inspect the distribution of abundance estimates.
Assembly: GRCm39
Supplementary files format and content: gene_expression_fpkm.txt
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| Submission date |
Mar 15, 2024 |
| Last update date |
Mar 18, 2024 |
| Contact name |
sungguan hong |
| E-mail(s) |
sungguanhong@cau.ac.kr
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| Organization name |
Chung-Ang
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| Department |
Chemistry
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| Street address |
84, Heukseok-ro, Dongjak-gu, Seoul, 104-407
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| City |
seoul |
| State/province |
none |
| ZIP/Postal code |
06974 |
| Country |
South Korea |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE261723 |
3M-Brazzein, a Natural Sugar Substitute, can Suppress Metabolic Disorders, NAFLD, and Depression-like Behaviors in Diabetic Mice |
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| Relations |
| BioSample |
SAMN40474919 |
| SRA |
SRX23964467 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM8149649_Het-W-L1.txt.gz |
191.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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