|
| Status |
Public on Oct 12, 2011 |
| Title |
placebo_Day14_311 |
| Sample type |
RNA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
drug administration: placebo
route of administration: subcutaneous
time point: Day14
batch: D
|
| Treatment protocol |
Blood was collected from each volunteer in PAXgene Blood RNA tubes for whole-blood RNA isolation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. RNA quantification was performed using a spectrophotometer (NanoDrop Technologies) and RNA quality was assessed using the Experion automated electrophoresis system (Bio-Rad).
|
| Label |
Biotin
|
| Label protocol |
Total RNA was amplified and labeled using the Illumina TotalPrep RNA Amplification kit, which is based on the Eberwine amplification protocol.
|
| |
|
| Hybridization protocol |
The biotinylated cRNA was hybridized onto Illumina Human RefSeq-8 BeadChips V3 at 58oC for 20 hrs
|
| Scan protocol |
Fluoresent array images were collected using Illumina BeadStation 500GX scanner and image intensity data were extracted using Illumina BeadStudio v3.
|
| Description |
5294095033_G
Blood collected from volunteer in PAXgene Blood RNA tubes at Day14 folowing placebo administration
|
| Data processing |
Illumina probe data were exported from BeadStudio as raw data and were screened for quality. Samples failing chip visual inspection and control examination were removed.Using the R software, the probe intensities were quantile-normalized followed by a log2 transformation and a batch effect subtraction using the ComBat procedure. The Y chromosome probes were filtred out
|
| |
|
| Submission date |
Oct 11, 2011 |
| Last update date |
Oct 12, 2011 |
| Contact name |
Ali Filali |
| E-mail(s) |
afilali@vgtifl.org
|
| Organization name |
Vaccine & Gene Therapy Institute of Florida
|
| Street address |
11350 SW Village Parkway, 3rd Floor
|
| City |
port st-lucie |
| State/province |
Florida |
| ZIP/Postal code |
34987 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE32862 |
Synthetic double stranded RNA reliably induces innate immunity similar to a live viral vaccine in humans |
|
