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| Status |
Public on Oct 12, 2011 |
| Title |
Synthetic double stranded RNA reliably induces innate immunity similar to a live viral vaccine in humans |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Adjuvants are critical for the success of vaccines, and agonists for microbial pattern recognition receptors are promising new candidates. A mechanism for the immune enhancing role of adjuvants is to stimulate innate immunity. We studied the innate immune response in humans to synthetic double stranded RNA (poly ICLC), a ligand for TLR3 and MDA-5 cytosolic RNA helicase. Transcriptional analysis of blood samples from eight volunteers, after subcutaneous administration of poly ICLC showed upregulation of genes involved in multiple innate immune pathways in all subjects, including interferon and inflammasome signaling. Blocking of type I interferon receptor ex vivo significantly dampened the response to poly IC. Comparative transcriptional analysis showed that several innate pathways were similarly induced in volunteers immunized with the highly efficacious Yellow Fever Vaccine. Therefore a chemically defined microbial agonist like poly ICLC can be a reliable and authentic microbial mimic for inducing innate immunity, here for a live attenuated viral vaccine in humans.
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| Overall design |
GSM813292-GSM813386: RNA expression obtained at different time points from Human blood after poly ICLC administration compared to RNA expression obtained from Human blood after placebo administration
GSM813387-GSM813410: Blocking of type I interferon receptor ex vivo followed by poly IC stimulation
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| Contributor(s) |
Caskey M, Lefebvre F, Filali-Mouhim A, Cameron MJ, Goulet J, Haddad EK, Breton G, Trumpfheller C, Pollak S, Shimeliovich I, Duque-Alarcon A, Pan L, Nelkenbaum A, Salazar AM, Schlesinger SJ, Steinman RM, Sékaly RP |
| Citation(s) |
22065672 |
| Submission date |
Oct 11, 2011 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Ali Filali |
| E-mail(s) |
afilali@vgtifl.org
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| Organization name |
Vaccine & Gene Therapy Institute of Florida
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| Street address |
11350 SW Village Parkway, 3rd Floor
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| City |
port st-lucie |
| State/province |
Florida |
| ZIP/Postal code |
34987 |
| Country |
USA |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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| Samples (119)
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| Relations |
| BioProject |
PRJNA146547 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32862_RAW.tar |
23.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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