|
| Status |
Public on Jan 01, 2012 |
| Title |
MCF-7_E2-BSA+G15 |
| Sample type |
RNA |
| |
|
| Source name |
treated for 3 hours with 10-6M E2-BSA plus 10-5M G15
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
cell type: breast cancer derived cells
|
| Treatment protocol |
MCF-7 cells were incubated with or without Estradiol, E2-BSA or combinations of E2-BSA with ICI or G15 for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS.
|
| Growth protocol |
The human breast cancer cell line MCF-7 was obtained from DSMZ (Braunschweig, Germany) and cultured in DMEM/F12 (1:1) supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual).
|
| |
|
| Hybridization protocol |
RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
|
| Scan protocol |
Signals were detected by an Affymetrix microarray chip reader.
|
| Description |
E2-BSA plus G15 treated cells 3h
|
| Data processing |
Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA).
|
| |
|
| Submission date |
Oct 06, 2011 |
| Last update date |
Jan 01, 2012 |
| Contact name |
George Notas |
| E-mail(s) |
gnotas@uoc.gr
|
| Organization name |
University of Crete
|
| Department |
Medicine
|
| Lab |
Experimental Endocrinology
|
| Street address |
PO BOX 2208
|
| City |
Heraklion |
| ZIP/Postal code |
71003 |
| Country |
Greece |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE32667 |
Time-course effect of estradiol and estradiol-BSA on early gene expression in MCF-7 cells |
| GSE32670 |
Time-course effect of estradiol and estradiol-BSA on early gene expression |
|
