Total RNA was extracted with TRIzol reagent from LV segments, and septal and lateral isolates from the 4 dyssynchronous mice were kept separate. RNA from the synchronous mice were pooled into a control septal and lateral sample. Functional genomic analysis was conducted on these 10 RNA samples with fluorophore reversal, such that each sample was assayed on two different microarrays. Corresponding septal and lateral samples from the same heart were paired on the same microarray to enhance signal/noise for assessing relative gene expression.
