|
| Status |
Public on Feb 29, 2024 |
| Title |
Fibroblasts,skin,C3,positive |
| Sample type |
SRA |
| |
|
| Source name |
skin
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skin
cell line: Fibroblasts
treatment: positive
|
| Treatment protocol |
Cells were positively or negatively selected using Anti-fibroblasts magnetic beads.
|
| Growth protocol |
MFs and SFs were isolated from meninges and skin fragments derived from 6 human brain donors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from MFs and SFs using Trizol® reagent. The specifications from the manufacturer were followed.
Lexogen CORALL Total RNA-seq Library Prep Kit, using 500 ng total RNA. Qualities of sequencing libraries were assessed with 4200 TapeStation with the D1000 High sensitivity Kit. RNA processing has been carried out using Illumina NextSeq 550 Sequencing.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
BB282_C_POS_S12
|
| Data processing |
FastQ files were generated via llumina bcl2fastq2 Version 2.17.1.14
Gene and transcript intensities were computed using STAR/RSEM software using Gencode Release m24 (GRCm38) as a reference, using the “-strandness forward” option.
Differental expression analysis for mRNA and lncRNA was performed using R package DESeq2
Assembly: HG19
Supplementary files format and content: fastq.gz
|
| |
|
| Submission date |
Feb 13, 2024 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Matteo Bordoni |
| Organization name |
IRCCS Mondino Foundation
|
| Street address |
Via Mondino 2
|
| City |
Pavia |
| ZIP/Postal code |
27100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL21697 |
| Series (1) |
| GSE255684 |
Functional analysis and transcriptome profile of meninges and skin fibroblasts from human aged donors |
|
| Relations |
| BioSample |
SAMN39938570 |
| SRA |
SRX23617765 |
