|
| Status |
Public on Oct 04, 2011 |
| Title |
Fib-1 |
| Sample type |
RNA |
| |
|
| Source name |
fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Fib-1
|
| Treatment protocol |
No special treatment during the culture.
|
| Growth protocol |
human iPSCs were cultured on Matrigel using mTeSR1 medium. Media were changed everyday.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were trypsinized and feeder cells were eliminated. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Whole Transcript (WT) Sense Target Labeling Assay Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
CNS-fibroblast_LW-1001
Gene expression data from Fib-1
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using Partek software.
|
| |
|
| Submission date |
Oct 03, 2011 |
| Last update date |
Oct 04, 2011 |
| Contact name |
wang xianming |
| E-mail(s) |
wang_xianming@gibh.ac.cn
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
|
| Street address |
kaiyuandadao190
|
| City |
guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE32527 |
DNA microarrays of Turner Syndrome induced pluripotent stem cells |
|
