|
| Status |
Public on Nov 01, 2011 |
| Title |
Infant at timeA, rep83 |
| Sample type |
RNA |
| |
|
| Source name |
Infant at 4-6 days of life rep 83
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
days of life: 6
gender: 1 (female)
gestational age (weeks): 26
birth weight (grams): 710
oxygen 28 days: 1 (oxygen therapy =>28 days)
oxygen 36 weeks postmenstrual age (wpma): 1 (on oxygen at the age of 36 wpma)
bronchopulmonary dysplasia (bpd) group: 3 (severe BPD)
retinopathy of prematurity (rop) group: 2 (ROP which need laser therapy)
rop lasertherapy: 1 (laser therapy due to prethreshold or threshold ROP)
periventricular leukomalacia (pvl): 0 (no PVL)
|
| Treatment protocol |
Blood samples (0.3 ml) were drawn from all the study participants on the 5th, 14th and 28th day of life for the assessment of whole genome expression in peripheral blood leukocytes.
|
| Growth protocol |
Samples were collected from children in the Neonatal Intensive Care Unit of the University Children's Hospital
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ficoll isopaque gradient centrifugation and RiboPure Blood Kit (Ambion, Life Technologies, Carlsbad, USA) were used for total RNA extraction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
GeneChip Hybridization Oven, 16-18 hours, 45oC, 60 rotations/min; washing and staining on Fluidics Station 450, protocol FS450_0007
|
| Scan protocol |
GeneChip Scanner 3000 7G controlled by GeneChip Operating Software
|
| Data processing |
Robust multiarray average (R/Bioconductor, using the library aroma.affymetrix)
|
| |
|
| Submission date |
Sep 29, 2011 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Ståle Nygård |
| E-mail(s) |
stale.nygard@medisin.uio.no
|
| Phone |
+47 95129127
|
| Organization name |
University of Oslo
|
| Street address |
Institute for Experimental Medical Research, Oslo University Hospital - Ulleval
|
| City |
Oslo |
| ZIP/Postal code |
0407 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE32472 |
Oxygen induced complication of prematurity: from experimental data to prevention strategy |
|
