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Sample GSM803335 Query DataSets for GSM803335
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_SK-N-SH_NRSF_PCR2x
Sample type SRA
Source name SK-N-SH
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell description: neuroblastoma, newly promoted to tier 2: not in 2011 analysis, the SK-N-SH line was established from a neuroblastoma in a 4 year old individual.
antibody antibodydescription: Mouse monoclonal. Antibody Target: NRSF
antibody targetdescription: Neuron-restrictive silencer transcription factor
antibody vendorname: Dr. David Anderson at Caltech
antibody vendorid: (Made by Protein Expression Center:
protocol description: a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
controlid: SL108,SL410
labexpid: SL83,SL409
replicate: 1,2
softwareversion: MACS
antibody targetdescription: Neuron-restrictive silencer transcription factor
antibody vendorname: David Anderson, Caltech
antibody vendorid: (Made by Protein Expression Center:
treatment: None
treatment description: No special treatment or protocol applies
protocol: PCR2x
protocol description: 2-cycles of PCR (Myers)
controlid: SL108
labexpid: SL83
replicate: 1
replicate description: tier 1
Biomaterial provider ATCC
Treatment protocol None
Growth protocol SK-N-SH_Myers_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
Data processing
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platform ID GPL9052
Series (2)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
SRA SRX100380
BioSample SAMN00738297
Named Annotation GSM803335_hg19_wgEncodeHaibTfbsSknshNrsfPcr2xRawRep1.bigWig
Named Annotation GSM803335_hg19_wgEncodeHaibTfbsSknshNrsfPcr2xRawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803335_hg19_wgEncodeHaibTfbsSknshNrsfPcr2xPkRep1.broadPeak.gz 24.2 Kb (ftp)(http) BROADPEAK
GSM803335_hg19_wgEncodeHaibTfbsSknshNrsfPcr2xPkRep2.broadPeak.gz 231.5 Kb (ftp)(http) BROADPEAK
GSM803335_hg19_wgEncodeHaibTfbsSknshNrsfPcr2xRawRep1.bigWig 10.6 Mb (ftp)(http) BIGWIG
GSM803335_hg19_wgEncodeHaibTfbsSknshNrsfPcr2xRawRep2.bigWig 36.3 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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