|
| Status |
Public on Jan 08, 2024 |
| Title |
R9Q_replicate_2 |
| Sample type |
SRA |
| |
|
| Source name |
whole animal
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: whole animal
genotype: unc-104(R9Q)
treatment: no
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Animal were lysed in 400μL TRIzol reagent (Invitrogen). Total RNA was extracted according to the manufacturer’s protocol.
library was constructed using KAPA RNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
The raw sequencing reads were trimmed using Trim_galore (version 0.4.4) to remove the low-quality bases and adaptor sequences
Paired-end reads with at least 20 nucleotides long were aligned to C. elegans reference genome (ce11) using STAR (2.5.4b).
The numbers of reads that aligned to genes were quantified by HTSeq (version 0.9.1).
Assembly: ce11
Supplementary files format and content: tab-delimited text file includes raw counts for each sample
|
| |
|
| Submission date |
Jan 07, 2024 |
| Last update date |
Jan 08, 2024 |
| Contact name |
Yongping Chai |
| Organization name |
Tsinghua University 清华大学
|
| Street address |
Tsinghua University
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100080 |
| Country |
China |
| |
|
| Platform ID |
GPL18730 |
| Series (1) |
| GSE252693 |
A Plant Flavonol and Genetic Suppressors Rescue a Pathogenic Mutation Associated with Kinesin in Neurons |
|
| Relations |
| BioSample |
SAMN39287385 |
| SRA |
SRX23122429 |
