|
| Status |
Public on Apr 30, 2012 |
| Title |
hESCs (rep 2) |
| Sample type |
RNA |
| |
|
| Source name |
H1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1
age: Passage 55
cell type: hESCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
| Label |
biotin
|
| Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using rank invariant normalisation with BeadStudio version 3 (Illumina)
|
| |
|
| Submission date |
Sep 07, 2011 |
| Last update date |
Apr 30, 2012 |
| Contact name |
Ying Wang |
| E-mail(s) |
wang_y@molgen.mpg.de
|
| Phone |
00493084131237
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Vertebrate Genomics
|
| Street address |
Ihnestrasse 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE31970 |
Molecular Insights into Non-teratoma-forming Induced Pluripotent Stem Cells |
|
