|
| Status |
Public on Apr 30, 2012 |
| Title |
Molecular Insights into Non-teratoma-forming Induced Pluripotent Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human induced pluripotent stem (iPS) cells are capable of differentiating into derivatives of the three embryonic germ layers both in vitro and in vivo. To date the the molecular differences between teratoma-forming cells and non-teratoma-forming cells has not been analysed. A cell line, B1, bears typical ES cell-like morphology, expression of pluripotency-associated genes, and in vitro pluripotency capacity, but fails to form teratomas after subcutaneously injected into immune-deficient mice based on histological analysis. Besides histological analysis, we characterized the tumors derived from line B1, and teratomas derived from bona fida iPS and ES (line H1) cells respectively, using microarray-based gene expression analysis. The expression levels of pluripotency-associated markers in B1 cells were comparable to that in iPS and ES cells, while the complexity of tissue expression commitment was decreased upon spontaneous differentiation of B1 cells as compared to iPS and ES cells.
|
| |
|
| Overall design |
Total RNA obtained from HFF1 (human foreskin fibroblast) cells, line B1, iPS-A4, iPS-B4 and ES (line H1) cells, and their derived tumors in immune-deficient mice.
|
| |
|
| Contributor(s) |
Ying W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 07, 2011 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Ying Wang |
| E-mail(s) |
wang_y@molgen.mpg.de
|
| Phone |
00493084131237
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Vertebrate Genomics
|
| Street address |
Ihnestrasse 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA144975 |
Less...
More...