|
| Status |
Public on Dec 21, 2005 |
| Title |
Mouse embryonic fibroblasts 15.5E wild type vs Wrn helicase mutant |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mouse embryonic fibroblasts, 15.5 days embryos, wild type
|
| Organism |
Mus musculus |
| Characteristics |
Strain: 129/Sv-Black Swiss wild type
Pool of three healthy embryos
mouse embryonic fibroblasts derived from embryonic tissues
|
| Biomaterial provider |
Laboratory of Michel Lebel
|
| Growth protocol |
Mouse embryonic cells were maintained in DMEM, 10% calf serum, 1% pen/strep. Cytoplasmic RNA was extracted at the second passage in culture.
|
| Extracted molecule |
cytoplasmic RNA |
| Extraction protocol |
Sambrook et al., 1989. Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press.
|
| Label |
Cy5
|
| Label protocol |
Protocol from Agilent
|
| |
|
| Channel 2 |
| Source name |
mouse embryonic fibroblasts, 15.5 days embryos, Wrn helicase in-frame deletion
|
| Organism |
Mus musculus |
| Characteristics |
Strain: 129/Sv-Black Swiss Wrn helicase in-frame deletion mutant
Pool of three healthy embryos
mouse embryonic fibroblasts derived from embryonic tissues
|
| Biomaterial provider |
Laboratory of Michel Lebel
|
| Growth protocol |
Mouse embryonic cells were maintained in DMEM, 10% calf serum, 1% pen/strep. Cytoplasmic RNA was extracted at the second passage in culture.
|
| Extracted molecule |
cytoplasmic RNA |
| Extraction protocol |
Sambrook et al., 1989. Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press.
|
| Label |
Cy3
|
| Label protocol |
Protocol from Agilent
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing was performed using the in situ Hybridization Plus kit (Agilent) and following the manufactures instructions.
|
| Scan protocol |
The arrays were scanned using a dual-laser DNA microarray scanner (Agilent). The data was then extracted from images by the Feature Extraction software 6.1 (Agilent).
|
| Description |
N/A
|
| Data processing |
The GeneSpring software (Agilent) was used to generate lists of selected genes and for different statistical methods. An Intensity-Dependent Normalization (known as Lowess normalization) was applied to correct for artifacts caused by nonlinear rates of dye incorporation as well as inconsistencies of the relative fluorescence intensity between some red and green dyes.
|
| |
|
| Submission date |
Sep 23, 2005 |
| Last update date |
Dec 21, 2005 |
| Contact name |
Michel Lebel |
| E-mail(s) |
michel.lebel@crchudequebec.ulaval.ca
|
| Phone |
(418) 525-4444
|
| Organization name |
Centre de Recherche du CHU de Québec
|
| Department |
Centre Hospitalier de l’Université Laval (CHUL)
|
| Lab |
Michel Lebel
|
| Street address |
2705 Laurier Blvd, Local T-2-04
|
| City |
Quebec |
| State/province |
Qc |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL891 |
| Series (1) |
| GSE3359 |
Differential expression of genes in cells mutant for Wrn and/or PARP-1 compared to wild type cells |
|
