|
| Status |
Public on Aug 01, 2011 |
| Title |
killifish hatchling_25ppm arsenic_rep11 |
| Sample type |
RNA |
| |
|
| Source name |
Killifish hatchling, 25ppm arsenic exposure
|
| Organism |
Fundulus heteroclitus |
| Characteristics |
developmental stage: <24hr post hatch
|
| Treatment protocol |
Fertilized eggs were exposed to 0 or 25ppm arsenic in water until hatch
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Mini
|
| Label |
Cy3
|
| Label protocol |
Agilent Quick Amp labeling kit, One-Color Cy3
|
| |
|
| Hybridization protocol |
Agilent One-Color Microarray-Based Gene Expression Analysis Hybridization Protocol
|
| Scan protocol |
Agilent G2505B Scanner, Agilent Feature Extraction V 10.7.3.1, Agilent Feature Extraction Protocol GE1_107_Sep09
|
| Data processing |
The background subtracted intensity values from each scan was subjected to variance stabilized normalization (VSN)
|
| |
|
| Submission date |
Jun 15, 2011 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Lisa J Bain |
| E-mail(s) |
lbain@clemson.edu
|
| Organization name |
Clemson University
|
| Department |
Biological Sciences
|
| Street address |
P. O. Box 709
|
| City |
Pendleton |
| State/province |
SC |
| ZIP/Postal code |
29672 |
| Country |
USA |
| |
|
| Platform ID |
GPL13723 |
| Series (1) |
| GSE29976 |
Fundulus exposed to arsenic as embryos |
|
