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| Status |
Public on Aug 01, 2011 |
| Title |
Fundulus exposed to arsenic as embryos |
| Organism |
Fundulus heteroclitus |
| Experiment type |
Expression profiling by array
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| Summary |
The present study used microarray expression profiling to determine the effects of embryonic arsenic exposure. Fertilized killifish (Fundulus heteroclitus) eggs were exposed to 0, 5, 15, or 25ppm arsenic as sodium arsenite. To examine differentially expressed genes, the microarrays were probed using RNA obtained from the control and 25ppm-exposed killifish just after hatching. No differences were noted in survival or hatching success between any of the groups. After analysis, a set of 332 genes was found to accurately distinguish between the control and 25ppm exposure groups. Expression of several of the genes (CDBP1, Arts1, FetB, and Fbp7) was quantified by qPCR in the lower exposure groups and at earlier time points to examine temporal and dose-responsive expression patterns. These results will enable us to better understand how arsenic impacts development.
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| Overall design |
Killifish eggs were fertilized, divided into petri dishes containing 40 eggs (n=10 replicate petri dishes), and cultured until hatch in 0 or 25 ppm arsenic as sodium arsenite. Four to five hatchlings within each petri dish were pooled to obtain RNA. A total of 20 arrays were probed, 10 with RNA from control fish and 10 with RNA from the arsenic-exposed fish.
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| Contributor(s) |
Gaworecki KM, Chapman RW, Beal M, Chapman RW, Bain LJ |
| Citation(s) |
22058191 |
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| Submission date |
Jun 15, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Lisa J Bain |
| E-mail(s) |
lbain@clemson.edu
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| Organization name |
Clemson University
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| Department |
Biological Sciences
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| Street address |
P. O. Box 709
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| City |
Pendleton |
| State/province |
SC |
| ZIP/Postal code |
29672 |
| Country |
USA |
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| Platforms (1) |
| GPL13723 |
Agilent-026181 Fundulus heteroclitus 45K (Probe name version) |
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| Samples (20)
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| GSM741991 |
killifish hatchling_25ppm arsenic_rep1 |
| GSM741992 |
killifish hatchling_25ppm arsenic_rep3 |
| GSM741993 |
killifish hatchling_25ppm arsenic_rep14 |
| GSM741994 |
killifish hatchling_25ppm arsenic_rep5 |
| GSM741995 |
killifish hatchling_25ppm arsenic_rep6 |
| GSM741996 |
killifish hatchling_25ppm arsenic_rep7 |
| GSM741997 |
killifish hatchling_25ppm arsenic_rep8 |
| GSM741998 |
killifish hatchling_25ppm arsenic_rep19 |
| GSM741999 |
killifish hatchling_25ppm arsenic_rep10 |
| GSM742000 |
killifish hatchling_25ppm arsenic_rep11 |
| GSM742001 |
killifish hatchling_control_rep1 |
| GSM742002 |
killifish hatchling_control_rep2 |
| GSM742003 |
killifish hatchling_control_rep3 |
| GSM742004 |
killifish hatchling_control_rep4 |
| GSM742005 |
killifish hatchling_control_rep5 |
| GSM742006 |
killifish hatchling_control_rep6 |
| GSM742007 |
killifish hatchling_control_rep7 |
| GSM742008 |
killifish hatchling_control_rep8 |
| GSM742009 |
killifish hatchling_control_rep9 |
| GSM742010 |
killifish hatchling_control_rep11 |
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| Relations |
| BioProject |
PRJNA140861 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29976_RAW.tar |
140.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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