|
| Status |
Public on May 07, 2012 |
| Title |
FLASH siRNA 10 hrs flash.1.10.jcp |
| Sample type |
RNA |
| |
|
| Source name |
SW480
|
| Organism |
Homo sapiens |
| Characteristics |
time point: 10 hrs
cell line: SW480
treatment: FLASH siRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) + RNase-Free DNase Set (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Quick Amp Labeling Kit (Agilent Technologies, Cat. #: 5190-0442), including MMLV-RT and oligo dT-Promoter primers for cDNA sinthesis, and T7 RNA Polymerase, Cy3-CTP and NTPs for labeling.
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit (Agilent Technologies, Cat. #: 5188-5242). Hybridizations were performed on SureHyb chambers on a rotator oven at 65º C for 17 hours.
|
| Scan protocol |
Agilent G2565BA
|
| Description |
aneuploid, colon adenocarcinoma cell line
|
| Data processing |
log2 transformation of signal intensity, quantile normalization
|
| |
|
| Submission date |
May 19, 2011 |
| Last update date |
May 07, 2012 |
| Contact name |
Frank Kramer |
| E-mail(s) |
frank.kramer@med.uni-goettingen.de
|
| Organization name |
University Medical Center Göttingen
|
| Department |
Department of Medical Statistics
|
| Street address |
Humboldtallee 32
|
| City |
Goettingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE29405 |
Systems-wide RNAi analysis of CASP8AP2 / FLASH |
|
