|
| Status |
Public on Aug 26, 2006 |
| Title |
FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
B cells - wildtype mice - IFN-b stimulated
|
| Organism |
Mus musculus |
| Characteristics |
Strain: 129SV/EV
Gender: Female
Age: 8-12weeks
Tissue: Lymph node B cell
17h treatment IFN-beta (2000U/ml)
|
| Biomaterial provider |
B&K Universal Ltd.- U. K.
|
| Treatment protocol |
In vitro exposure to IFN-b (17 hours, 2000U/ml)
|
| Growth protocol |
Mice were bred and held under conventional housing conditions in filtertop cages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Bioarray (Enzo)
|
| |
|
| Hybridization protocol |
Hybridized washed and stained with streptavidin-PE per the manufacturer's protocol (Affymetrix).
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 and GCOS software using target value = 262
|
| Description |
B cells from wild type mice treated with IFN-b
Array 23 - 23.N.B._565.CEL
|
| Data processing |
The dataset was preprocessed using the following procedures: (1) background correction; (2) normalization using the quantile method (Bolstad et al. 2003); (3) summarization of probe set values using the RMA (Robust Multi-array Analysis) method, which fits a specified robust linear model to the probe level data. The abovementioned procedures were performed using the Bioconductor package “affyPLM”.
|
| |
|
| Submission date |
Aug 25, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Michael E. Salazar |
| Phone |
(415) 514-4371
|
| URL |
http://arrays.ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Department |
|
| Lab |
Functional Genomics Core Laboratories
|
| Street address |
1550 Fourth Street, RM 545
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE3203 |
Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN |
|
| Relations |
| Reanalyzed by |
GSE119085 |
