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| Status |
Public on Aug 26, 2006 |
| Title |
Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Influenza virus infection-induced gene expression changes of regional B cells are mediated at least in part through type I Interferon:
Our objective is to determine whether the influenza virus-infection induced gene expression changes in regional lymph node B cells are facilitated at least in part through type I interferon. Our specific aim is to compare the gene expression profile of highly FACS-purified B cells in the regional lymph nodes of wildtype and IFNR-/- mice prior to and 48h following infection with influenza virus infection and to contrast this expression profile with that of FACS-purified wildtype B cells activated in vitro with IFN-beta +/- anti-CD86 for 12h.
Keywords: Infection-induced responses
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| Overall design |
We analyzed gene expression from mouse lymph node B cells purified by flow cytometric sorting using single channel oligonucleotide microarrays. There were 4 groups: 1) wild type uninfected mice (control group), 2) wild type mice infected with influenza (flu) for 2 days, 3) IFNR-/- mice infected with flu for 2 days, 4) cells stimulated with IFN-b in vitro for 17 h. Each group contained 4 biological replicates obtained from independent experiments. There were 16 total samples and each was measured on a separate array.
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| Contributor(s) |
Coro ES, Chang WL, Doucett VP, Xiao Y, Erle DJ, Baumgarth N |
| Citation(s) |
17237394 |
| |
| Submission date |
Aug 25, 2005 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Michael E. Salazar |
| Phone |
(415) 514-4371
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| URL |
http://arrays.ucsf.edu
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| Organization name |
University of California, San Francisco
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| Department |
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| Lab |
Functional Genomics Core Laboratories
|
| Street address |
1550 Fourth Street, RM 545
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (16)
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| GSM71992 |
FACS-purified B cells_wildtype_non-infected_rep1 |
| GSM71993 |
FACS-purified B cells_wildtype_non-infected_rep2 |
| GSM71994 |
FACS-purified B cells_129SV/EV_non-infected_rep3 |
| GSM71995 |
FACS-purified B cells_129SV/EV_non-infected_rep4 |
| GSM71996 |
FACS-purified B cells_wildtype_influenza-infected_rep1 |
| GSM71997 |
FACS-purified B cells_129SV/EV_influenza-infected_rep2 |
| GSM71998 |
FACS-purified B cells_129SV/EV_influenza-infected_rep3 |
| GSM71999 |
FACS-purified B cells_129SV/EV_influenza-infected_rep4 |
| GSM72000 |
FACS-purified B cells_IFNAR1-/-_influenza-infected_rep1 |
| GSM72001 |
FACS-purified B cells_IFNAR1-/-_influenza-infected_rep2 |
| GSM72002 |
FACS-purified B cells_IFNAR1-/-_influenza-infected_rep3 |
| GSM72003 |
FACS-purified B cells_IFNAR1-/-_influenza-infected_rep4 |
| GSM72004 |
FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep1 |
| GSM72005 |
FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep2 |
| GSM72006 |
FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep3 |
| GSM72007 |
FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep4 |
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| Relations |
| BioProject |
PRJNA92871 |
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