|
| Status |
Public on Mar 20, 2024 |
| Title |
Mouse Cardiac Endothelial Cell - Empty vector (EV) - rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Mouse Cardiac Endothelial Cell
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Mouse Cardiac Endothelial Cell
cell type: Mouse Cardiac Endothelial Cells
genotype: CTL
treatment: Empty vector lentivirus transduction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using miRNeasy Micro kit (Qiagen). 3 ug of total RNA was used for the construction of sequencing libraries.
llumina standard protocols
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Original image data file from high-throughput sequencing is transformed into sequenced reads (called Raw Data or Raw Reads) by CASAVA base recognition (Base Calling). Raw data are stored in FASTQ(fq) format files, which contain sequences of reads and corresponding base quality. Each read has four descriptive sentences in as follow:
For the samples with biological replicates: Differential expression analysis of two conditions/groups was performed using the DESeq R package (1.6.3). The padj<0.05 was set as the threshold for significantly differential expression by default
Assembly: mm10
|
| |
|
| Submission date |
Apr 13, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Deqiang Li |
| Organization name |
Nationwide Children's Hospital
|
| Lab |
Li Lab
|
| Street address |
575 Children’s Crossroad, WB4321
|
| City |
COLUMBUS |
| State/province |
Ohio |
| ZIP/Postal code |
43215 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE229659 |
Endocardial HDAC3 is required for myocardial trabeculation [miRNA-seq] |
| GSE229661 |
Endocardial HDAC3 is required for myocardial trabeculation |
|
| Relations |
| SRA |
SRX19960006 |
| BioSample |
SAMN34171943 |
