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Sample GSM710183 Query DataSets for GSM710183
Status Public on Apr 16, 2011
Title Th2_rep1
Sample type SRA
Source name Th2 cells
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: T helper cell type 2 (Th2)
Growth protocol Murine CD4+CD62L+ splenocytes were activated for 5 days with anti-CD3/CD28 in the presence of 10 ng/ml IL-4 and 2 ug/ml anti-IFN-g, followed by 3 days of resting.
Extracted molecule polyA RNA
Extraction protocol Poly(A)+ RNA was isolated from cells using the Oligotex Direct mRNA kit (Qiagen), followed by 1st and 2nd strand synthesis using the JustcDNA kit (Stratagene), followed by sonication, followed by library preparation using the Illumina ChIP-seq sample prep kit.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
Description Biological replicate 1 of 2.
Data processing Reads were mapped to mm9 with Bowtie -m 1 --best -strata --solexa1.3-quals
Submission date Apr 15, 2011
Last update date May 15, 2019
Contact name Daniel Hebenstreit
Organization name University of Warwick
Street address Gibbet Hill Rd
City Coventry
ZIP/Postal code CV47AL
Country United Kingdom
Platform ID GPL11002
Series (1)
GSE28666 RNA sequencing reveals two major classes of gene expression levels in metazoan cells
Reanalyzed by GSE80797
SRA SRX057856
BioSample SAMN00259740

Supplementary file Size Download File type/resource
GSM710183_Th2_rep1.bed.gz 112.1 Mb (ftp)(http) BED
GSM710183_Th2_rep1.bedgraph.gz 71.2 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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