 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 16, 2011 |
| Title |
RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The expression level of a gene is often used as a proxy for determining whether the protein or RNA product is functional in a cell or tissue. Therefore, it is of fundamental importance to understand the global distribution of gene expression levels, and to be able to interpret it mechanistically and functionally. Here we use RNA sequencing of mouse Th2 cells, coupled with a range of other techniques, to show that all genes can be separated, based on their expression abundance, into two distinct groups: one group comprising of lowly expressed and putatively non-functional mRNAs, and the other of highly expressed mRNAs with active chromatin marks at their promoters. Similar observations are made in other data sets, including sources such as Drosophila.
|
| |
|
| Overall design |
RNA-seq data of two biological replicates of murine Th2 cells.
|
| |
|
| Contributor(s) |
Hebenstreit D, Fang M, Gu M, Charoensawan V, van Oudenaarden A, Teichmann SA |
| Citation(s) |
21654674, 24813893 |
| Submission date |
Apr 15, 2011 |
| Last update date |
Oct 22, 2019 |
| Contact name |
Daniel Hebenstreit |
| E-mail(s) |
D.Hebenstreit@warwick.ac.uk
|
| Organization name |
University of Warwick
|
| Street address |
Gibbet Hill Rd
|
| City |
Coventry |
| ZIP/Postal code |
CV47AL |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| SRA |
SRP006435 |
| BioProject |
PRJNA138875 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28666_RAW.tar |
309.7 Mb |
(http)(custom) |
TAR (of BED, BEDGRAPH) |
| GSE28666_readme.txt |
417 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
