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Series GSE28666 Query DataSets for GSE28666
Status Public on Apr 16, 2011
Title RNA sequencing reveals two major classes of gene expression levels in metazoan cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The expression level of a gene is often used as a proxy for determining whether the protein or RNA product is functional in a cell or tissue. Therefore, it is of fundamental importance to understand the global distribution of gene expression levels, and to be able to interpret it mechanistically and functionally. Here we use RNA sequencing of mouse Th2 cells, coupled with a range of other techniques, to show that all genes can be separated, based on their expression abundance, into two distinct groups: one group comprising of lowly expressed and putatively non-functional mRNAs, and the other of highly expressed mRNAs with active chromatin marks at their promoters. Similar observations are made in other data sets, including sources such as Drosophila.
Overall design RNA-seq data of two biological replicates of murine Th2 cells.
Contributor(s) Hebenstreit D, Fang M, Gu M, Charoensawan V, van Oudenaarden A, Teichmann SA
Citation(s) 21654674, 24813893
Submission date Apr 15, 2011
Last update date Oct 22, 2019
Contact name Daniel Hebenstreit
Organization name University of Warwick
Street address Gibbet Hill Rd
City Coventry
ZIP/Postal code CV47AL
Country United Kingdom
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM710183 Th2_rep1
GSM710184 Th2_rep2
SRA SRP006435
BioProject PRJNA138875

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Supplementary file Size Download File type/resource
GSE28666_RAW.tar 309.7 Mb (http)(custom) TAR (of BED, BEDGRAPH)
GSE28666_readme.txt 417 b (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file

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