|
Status |
Public on Apr 06, 2011 |
Title |
Wildtype 2 |
Sample type |
RNA |
|
|
Source name |
MHCIIhi mTECs
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 genotype: wt beadchip: 1 Sex: male date: Sep-08
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using a Qiagen RNeasy mini kit including an on column DNase digest as per manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Standard Illumina protocol
|
Scan protocol |
Standard Illumina protocol
|
Data processing |
Un-normalized summary probe profiles were output from BeadStudio for two Illumina Mouse-6 Expression Beadchip (v1.1). The raw intensities were background corrected using the negative controls, a variance stabilising transformation (vst) was applied and the data quantile normalised using all the arrays on the two beadchips using the lumi package of the Bioconductor open-source software project (http://www.bioconductor.org).
|
|
|
Submission date |
Apr 05, 2011 |
Last update date |
Apr 06, 2011 |
Contact name |
Francois-Xavier Hubert |
E-mail(s) |
hubert@wehi.edu.au
|
Organization name |
The Walter and Eliza Hall Institute of Medical Research
|
Department |
Immunology
|
Street address |
1G Royal Parade
|
City |
Melbourne |
State/province |
Victoria |
ZIP/Postal code |
3052 |
Country |
Australia |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE28393 |
Aire regulates transfer of antigen from mTEC to dendritic cells for induction of thymic tolerance |
|