 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 06, 2011 |
| Title |
Aire regulates transfer of antigen from mTEC to dendritic cells for induction of thymic tolerance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate the role of Aire in thymic selection, we examined the cellular requirements for generation of ovalbumin (OVA)-specific CD4 and CD8 T cells in mice expressing OVA under the control of the rat insulin promoter (RIP). Aire-deficiency reduced the number of mature single positive (SP) OVA-specific CD4+ or CD8+ T cells in the thymus, independent of OVA expression. Importantly, it also contributed in two way to OVA-dependent negative selection depending on the T cell type. Aire-dependent negative selection of OVA-specific CD8 T cells correlated with Aire-regulated expression of OVA. By contrast, for OVA-specific CD4 T cells, Aire affected tolerance induction by a mechanism that operated independent of the level of OVA expression, controlling access of antigen presenting cells to mTEC-expressed OVA. This study supports the view that one mechanism by which Aire controls thymic negative selection is by regulating the indirect presentation of mTEC-derived antigens by thymic dendritic cells. It also indicates that mTEC can mediate tolerance by direct presentation of Aireregulated antigens to both CD4 and CD8 T cells.
|
| |
|
| Overall design |
Total RNA was extracted from medullary thymic epithelial cells isolated from wildtype and Aire-deficient C57BL/6 mice for comparison of gene expression profiles.
|
| |
|
| Contributor(s) |
Hubert F, Kinkel SA, Davey GM, Phipson B, Mueller SN, Liston A, Proietto AI, Cannon PZ, Forehan S, Smyth GK, Wu L, Goodnow CC, Carbone FR, Scott HS, Heath WR |
| Citation(s) |
21505196 |
| |
| Submission date |
Apr 05, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Francois-Xavier Hubert |
| E-mail(s) |
hubert@wehi.edu.au
|
| Organization name |
The Walter and Eliza Hall Institute of Medical Research
|
| Department |
Immunology
|
| Street address |
1G Royal Parade
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL6481 |
Illumina mouse-6 v1.1 expression beadchip [Array_Address_Id version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA139083 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28393_full_normalized_matrix.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| GSE28393_non-normalized.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...