|
| Status |
Public on Feb 22, 2012 |
| Title |
colon_INT_T1005_cardA |
| Sample type |
RNA |
| |
|
| Source name |
Colon, RNA
|
| Organism |
Homo sapiens |
| Characteristics |
type: T
patient: 9927424
Stage: II
status: NED
cea: 2.65
ca199: 57.48
apc: 1
kras: 0
chr18qloh: 1
tp53: 0
|
| Biomaterial provider |
Fondazione IRCCS Istituto Nazionale dei Tumori (INT)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacture's instructions.
|
| Label |
FAM dye
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
MicroRNA expression data of sporadic colon cancer patient 9927424
|
| Data processing |
The cycle threshold (Ct) is defined as the number of cycles required for the fluorescent signal to cross the threshold in qPCR and was quantified using the Sequence Detector System (SDS) software version 2.2.2 (Applied Biosystem, Foster City, CA). The undetermined data were set to the Ct value of 40. Data were normalized data using the mean U6 on each card (4 technical replicates).
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
Feb 22, 2012 |
| Contact name |
James F Reid |
| E-mail(s) |
james.reid@ifom-ieo-campus.it
|
| Organization name |
Fondazione Istituto FIRC di Oncologia Molecolare
|
| Department |
Molecular Genetics of Cancer
|
| Lab |
Cancer genomics
|
| Street address |
Via Adamello, 16
|
| City |
Milano |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
|
| Platform ID |
GPL13328 |
| Series (1) |
| GSE28364 |
MicroRNA profiling in colorectal cancer highlights miR-1 involvement in MET-dependent proliferation |
|
