|
| Status |
Public on Apr 01, 2011 |
| Title |
Gene expression in NRF2-knockdown A549 cells (no.2) |
| Sample type |
RNA |
| |
|
| Source name |
human lung cancer cell line, A549 cells, treated with NRF2 siRNA (HSS107129)
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: NRF2 KD-2
cell line: A549
|
| Treatment protocol |
The siRNAs were introduced into A549 cells by electroporation using the MP-100 MicroPorator (Digital Bio Technology).
|
| Growth protocol |
A549 cells were maintained in RPMI1640 medium supplemented with 20% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from A549 cells using Isogen (Nippon Gene). RNA was further purified using an RNeasy Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
500 ng of Total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
750 ng of Cy3-labeled cRNA was fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
|
| Scan protocol |
Slides were scanned with an Agilent DNA Microarray Scanner model G2539.
|
| Description |
Gene expression at 24 hours after transfection
|
| Data processing |
Agilent Feature Extraction Software
|
| |
|
| Submission date |
Mar 29, 2011 |
| Last update date |
Apr 07, 2011 |
| Contact name |
Hozumi Motohashi |
| E-mail(s) |
hozumim@med.tohoku.ac.jp
|
| Phone |
81-22-717-8087
|
| Fax |
81-22-717-8090
|
| Organization name |
Tohoku University Graduate School of Medicine
|
| Department |
Center for Radioisotope Science
|
| Street address |
2-1 Seiryo-machi, Aoba-ku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE28230 |
Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA |
|
