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Series GSE28230 Query DataSets for GSE28230
Status Public on Apr 01, 2011
Title Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To examine the role of NRF2 in accelerating cell proliferation and to identify the target genes responsible for this function, transcriptome analysis was performed using A549 cells, in which NRF2 is constitutively activated.
NRF2 was knocked down by siRNA against NRF2, and the gene expression profile was compared with that of A549 cells treated with control siRNA. To exclude off-target effects, three different siRNAs against NRF2 was independently applied.
Overall design NRF2 siRNA or control siRNA was transfected into A549 cells. Cells were harvested 24 hours after transfection, and total RNA was purified.
Contributor(s) Mitsuishi Y, Yamamoto M, Motohashi H
Citation(s) 22789539
Submission date Mar 29, 2011
Last update date Feb 22, 2018
Contact name Hozumi Motohashi
Phone 81-22-717-8087
Fax 81-22-717-8090
Organization name Tohoku University Graduate School of Medicine
Department Center for Radioisotope Science
Street address 2-1 Seiryo-machi, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (4)
GSM698671 Gene expression in NRF2-knockdown A549 cells (no.1)
GSM698672 Gene expression in NRF2-knockdown A549 cells (no.2)
GSM698673 Gene expression in NRF2-knockdown A549 cells (no.3)
BioProject PRJNA139597

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28230_RAW.tar 14.6 Mb (http)(custom) TAR (of TXT)
GSE28230_fold_change.txt.gz 58.0 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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