|
| Status |
Public on Mar 21, 2012 |
| Title |
heart_diabetic_failure_sample_27 |
| Sample type |
RNA |
| |
|
| Source name |
LEFT VENTRICLE, DIABETIC, HEART FAILURE
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: left ventricle cardiac biopsy taken from the vital, non-infarcted zone
gender: MALE
age: 60 years
disease state: DIABETIC, HEART FAILURE
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen) extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNAeasy mini kit following standard RNA cleanup protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA Ambion WT Expression Kit (Life Technologies Co.)
|
| |
|
| Hybridization protocol |
Following fragmentation, 30 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Description |
MALE, 60 years
|
| Data processing |
Summarized signal intensities were generated in BRB ArrayTools using justRMA
|
| |
|
| Submission date |
Jan 26, 2011 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Fabio Martelli |
| E-mail(s) |
fabio.martelli@grupposandonato.it
|
| Phone |
+390252774533
|
| Organization name |
IRCCS-Policlinico San Donato
|
| Lab |
Molecular Cardiology
|
| Street address |
via morandi 30
|
| City |
Milan |
| ZIP/Postal code |
20097 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE26887 |
Transcriptome analysis of diabetic and non diabetic patients affected by post-ischemic heart failure |
|
