|
| Status |
Public on Jan 21, 2011 |
| Title |
normal_5 |
| Sample type |
RNA |
| |
|
| Source name |
normal Patient 5
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: normal prostate
cell-type: dendritic cells
|
| Treatment protocol |
Cells were purified from digested prostates by magnetic beads >90% purity was confirmed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the qiagen RNAeasy mini spin colomn according to manufacturer's instuctions. All samples used for microarray analysis have high quality score (RIN >9).
RNA quality was checked on Agilent Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol
|
| |
|
| Hybridization protocol |
replicates of each group were hybridized to Affymetrixhuman Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs
|
| Scan protocol |
Wash and stain were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000
|
| Description |
Dendritic cells normal human prostate patient 5
|
| Data processing |
Data were collected using Affymetrix AGCC software,Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm.
|
| |
|
| Submission date |
Jan 20, 2011 |
| Last update date |
Jan 21, 2011 |
| Contact name |
Stephanie Kaye Watkins |
| E-mail(s) |
watkinssk@mail.nih.gov
|
| Phone |
301-846-6871
|
| Fax |
301-846-6005
|
| Organization name |
NCI-Frederick
|
| Department |
CIP/LMI
|
| Lab |
Tumor Immunity and Tolerance
|
| Street address |
1050 Boyles St.
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE26747 |
Gene expression analysis of dendritic cells from normal or tumor sections of human prostates |
|
