|
| Status |
Public on Dec 16, 2010 |
| Title |
Blood_DNA_Methylation_AsUnexposed_2 |
| Sample type |
genomic |
| |
|
| Source name |
Blood_No_Arsenicosis_Signs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
Sex: Female
age: Age58
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
QiagenDNA Extraction Kit. CpG-methylated DNA was pulled down using a methyl binding domain protein complex. Samples were labeled with the Affymetrix GeneChip WT dsDNA labeling kit and hybridized to Affymetrix Human Promoter 1.0R arrays.
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip WT dsDNA labeling kit (TdT)
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix Human Promoter 1.0R Array (GPL5082)
|
| Scan protocol |
Chips were scanned using Affymetrix scanner
|
| Description |
Platform GPL5082
|
| Data processing |
Data were analyzed with Robust multichip analysis in Partek Genomics Suite (version 6.4). RMA normalized data are provided as a supplementary file on the Series record.
|
| |
|
| Submission date |
Dec 15, 2010 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL5082 |
| Series (1) |
| GSE26073 |
DNA methylation patterns associated with arsenicosis |
|
