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| Status |
Public on Dec 16, 2010 |
| Title |
DNA methylation patterns associated with arsenicosis |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
DNA methylation patterns were analyzed in blood samples from humans unexposed or exposed to arsenic
Using a state-of-the-art technique to map the methylomes of our study subjects, we identified a large interactome of hypermethylated genes that are enriched for their involvement in arsenic-associated diseases, such as cancer, heart disease, and diabetes. Notably, we have uncovered an arsenic-induced “suppressorome” - a complex of 17 known and putative tumor suppressors silenced in human cancers. This finding represents a pivotal clue in unravelling a possible epigenetic mode of arsenic induced disease.
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| Overall design |
DNA was extracted from 16 blood samples, 8 of which were from individuals showing signs of arsenicosis and 8 from individuals not showing signs of arsenicosis. CpG-methylated DNA was pulled down using a methyl binding domain protein. DNA samples were hybridized to Affymetrix Human Promoter 1.0R arrays, and chips were scanned using an Affymetrix scanner.
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| Contributor(s) |
Smeester L, Rager JE, Bailey KA, Zhang L, Guan X, Smith N, Garcia-Vargas G, Del Razo L, Drobna Z, Kelkar H, Schroth G, Styblo M, Fry RC |
| Citation(s) |
21291286 |
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| Submission date |
Dec 15, 2010 |
| Last update date |
Jan 21, 2015 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
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| Organization name |
UNC-Chapel Hill
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| Department |
Environmental Sciences and Engineering
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| Street address |
1213 MHRC
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| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL5082 |
[Hs_PromPR] Affymetrix Human Promoter 1.0R Array |
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| Samples (16)
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| GSM640144 |
Blood_DNA_Methylation_AsUnexposed_1 |
| GSM640145 |
Blood_DNA_Methylation_AsUnexposed_2 |
| GSM640146 |
Blood_DNA_Methylation_AsUnexposed_3 |
| GSM640147 |
Blood_DNA_Methylation_AsUnexposed_4 |
| GSM640148 |
Blood_DNA_Methylation_AsUnexposed_5 |
| GSM640149 |
Blood_DNA_Methylation_AsUnexposed_6 |
| GSM640150 |
Blood_DNA_Methylation_AsUnexposed_7 |
| GSM640151 |
Blood_DNA_Methylation_AsUnexposed_8 |
| GSM640152 |
Blood_DNA_Methylation_AsExposed_9 |
| GSM640153 |
Blood_DNA_Methylation_AsExposed_10 |
| GSM640154 |
Blood_DNA_Methylation_AsExposed_11 |
| GSM640155 |
Blood_DNA_Methylation_AsExposed_12 |
| GSM640156 |
Blood_DNA_Methylation_AsExposed_13 |
| GSM640157 |
Blood_DNA_Methylation_AsExposed_14 |
| GSM640158 |
Blood_DNA_Methylation_AsExposed_15 |
| GSM640159 |
Blood_DNA_Methylation_AsExposed_16 |
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| Relations |
| BioProject |
PRJNA135201 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26073_MethylData_RMA_16arrays.txt.gz |
212.0 Mb |
(ftp)(http) |
TXT |
| GSE26073_RAW.tar |
304.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data are available on Series record |
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