|
Status |
Public on May 23, 2011 |
Title |
asf1-del-1-d |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
asf1-del
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: BY4742 genotype: asf1-del
|
Extracted molecule |
total RNA |
Extraction protocol |
yeast HTP RNA isolation for robot v2.0
|
Label |
Cy5
|
Label protocol |
robot amplification and labeling v1.0
|
|
|
Channel 2 |
Source name |
refpool
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: BY4742 genotype: wild type
|
Extracted molecule |
total RNA |
Extraction protocol |
yeast RNA isolation for robotic amplification v1.0
|
Label |
Cy3
|
Label protocol |
robot amplification and labeling v1.0
|
|
|
|
Hybridization protocol |
Tecan HS4800 hybridization
|
Scan protocol |
scanning of slides using Agilent G256BA Imagene feature extraction
|
Data processing |
as genes, no bg corr, but followed by gene-specific dye bias correction. Identical to genes, no bg corr if the dye bias correction is 0.0, which is the default. So far (jan 2008), we have only dye-bias corrections for yeast. limma, A software package for the analysis of gene expression microarray data, especially the use of linear models for analysing designed experiments and the assessment of differential expression. Author(s): Gordon Smyth. The limma R package version 2.12.0 is used. P-values are Benjamini-Hochberg FDR corrected.
|
|
|
Submission date |
Dec 07, 2010 |
Last update date |
May 23, 2011 |
Contact name |
Patrick Kemmeren |
Organization name |
UMC Utrecht
|
Department |
Department of Molecular Cancer Research
|
Lab |
Holstege Lab
|
Street address |
Universiteitsweg 100
|
City |
Utrecht |
State/province |
Utrecht |
ZIP/Postal code |
3584 CG |
Country |
Netherlands |
|
|
Platform ID |
GPL11232 |
Series (3) |
GSE25909 |
Gene deletion expression profiles of yeast chromatin modifiers |
GSE42527 |
Compendium of deletion mutant gene expression profiles, 700 responsive mutants [hs1991] |
GSE42528 |
Compendium of deletion mutant gene expression profiles |
|