|
| Status |
Public on Dec 07, 2010 |
| Title |
DU145 ST3-Hel2A-2-1 |
| Sample type |
RNA |
| |
|
| Source name |
DU145 treated with ST3-Hel2A-2 for 3hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145 prostate cancer cell line
agent: ST3-Hel2A-2
|
| Treatment protocol |
Cells were treated with ST3-Hel2A-2 or DMSO for 3 hr
|
| Growth protocol |
DU145 prostate cancer cell line was obtained from American Type Culture Collection. Cells were maintained in RPMI 1640 supplemented with 10% heat-inactivated FBS. Cell cultures were maintained at 37°C under a humidified atmosphere of 5% CO2 in an incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the Qiagen RNeasy manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from DU145 treated with ST3-Hel2A-2
|
| Data processing |
Matrix table was generated using BRB-ArrayTools Version: 4.1.0 - Beta_3 Release (October 2010) using GC-RMA as normalization method.
|
| |
|
| Submission date |
Dec 06, 2010 |
| Last update date |
Dec 09, 2010 |
| Contact name |
Olga A Timofeeva |
| E-mail(s) |
oat@georgetown.edu
|
| Phone |
202-687-8810
|
| Fax |
202-687-2221
|
| Organization name |
Georgetown University Medical Center
|
| Department |
Oncology
|
| Street address |
3900 Reservoir Rd NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20007 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE25866 |
Expression data from DU145 cells treated with ST3-Hel2A-2 STAT3 N-domain inhibitor coupled to analysis of genome-wide STAT3 binding sites |
| GSE25944 |
Role of STAT3 in DU145 prostate cancer cell line |
|
