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| Status |
Public on Dec 07, 2010 |
| Title |
Expression data from DU145 cells treated with ST3-Hel2A-2 STAT3 N-domain inhibitor coupled to analysis of genome-wide STAT3 binding sites |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Activation of Signal Transducer and Activator of Transcription 3 (STAT3) is common in prostate cancers. STAT3 may induce cell proliferation and resistance to apoptosis, as well as promote tumor angiogenesis, invasion, and migration by activating gene expression. Many STAT3-dependent transcriptional responses are mediated through protein-protein interactions that involve the amino-terminal domain (N-domain).
In this study, we found that inhibition of the STAT3 N-domain using novel inhibitor ST3-Hel2A-2 induces apoptotic death in prostate cancer cells. The cell death was accomponied by robust activation of pro-apoptotic gene. Using chromatin immunoprecipitation and tiling human promoter arrays (ChIP-chip), we have defined genome-wide targets of STAT3 in DU145 prostate cancer cells. We found that upregulated pro-apoptotic genes were bound by STAT3 in prostate cancer cells, and that STAT3 binding was decreased following inhibition of the STAT3 N-domain.
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| Overall design |
DU145 cells were treated with ST3-Hel2A-2 or DMSO as a control for 3 hr. Total RNA has been extracted and prepared for hybridization on Affymetrix HG-U133A 2.0 arrays.
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| Contributor(s) |
Timofeeva OA |
| Citation(s) |
23288901 |
| |
| Submission date |
Dec 06, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Olga A Timofeeva |
| E-mail(s) |
oat@georgetown.edu
|
| Phone |
202-687-8810
|
| Fax |
202-687-2221
|
| Organization name |
Georgetown University Medical Center
|
| Department |
Oncology
|
| Street address |
3900 Reservoir Rd NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20007 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE25944 |
Role of STAT3 in DU145 prostate cancer cell line |
|
| Relations |
| BioProject |
PRJNA142467 |
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