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Series GSE25866 Query DataSets for GSE25866
Status Public on Dec 07, 2010
Title Expression data from DU145 cells treated with ST3-Hel2A-2 STAT3 N-domain inhibitor coupled to analysis of genome-wide STAT3 binding sites
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Activation of Signal Transducer and Activator of Transcription 3 (STAT3) is common in prostate cancers. STAT3 may induce cell proliferation and resistance to apoptosis, as well as promote tumor angiogenesis, invasion, and migration by activating gene expression. Many STAT3-dependent transcriptional responses are mediated through protein-protein interactions that involve the amino-terminal domain (N-domain).
In this study, we found that inhibition of the STAT3 N-domain using novel inhibitor ST3-Hel2A-2 induces apoptotic death in prostate cancer cells. The cell death was accomponied by robust activation of pro-apoptotic gene. Using chromatin immunoprecipitation and tiling human promoter arrays (ChIP-chip), we have defined genome-wide targets of STAT3 in DU145 prostate cancer cells. We found that upregulated pro-apoptotic genes were bound by STAT3 in prostate cancer cells, and that STAT3 binding was decreased following inhibition of the STAT3 N-domain.
Overall design DU145 cells were treated with ST3-Hel2A-2 or DMSO as a control for 3 hr. Total RNA has been extracted and prepared for hybridization on Affymetrix HG-U133A 2.0 arrays.
Contributor(s) Timofeeva OA
Citation(s) 23288901
Submission date Dec 06, 2010
Last update date Dec 06, 2018
Contact name Olga A Timofeeva
Phone 202-687-8810
Fax 202-687-2221
Organization name Georgetown University Medical Center
Department Oncology
Street address 3900 Reservoir Rd NW
City Washington
State/province DC
ZIP/Postal code 20007
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (6)
GSM635119 DU145 control-1
GSM635120 DU145 control-2
GSM635121 DU145 control-3
This SubSeries is part of SuperSeries:
GSE25944 Role of STAT3 in DU145 prostate cancer cell line
BioProject PRJNA142467

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25866_RAW.tar 11.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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