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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 17, 2024 |
| Title |
Microglia, Vehicle, biol rep 1 |
| Sample type |
SRA |
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| Source name |
Primary Microglia
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| Organism |
Mus musculus |
| Characteristics |
cell type: Primary Microglia
strain: CD1
treatment: Vehicle
time: 24h
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| Extracted molecule |
total RNA |
| Extraction protocol |
Microglia RNA was extracted using the RNeasy mini kit (Qiagen) following the manufacturer’s instructions, including on column DNase digestion
Directional mRNA library preparation (PolyA enrichment)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
40M reads per sample
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| Data processing |
Data preprocessing performed by Novogene UK, including filtering of raw reads for adapter contamination and low-quality reads
Sequence alignment was performed with HISAT2 to map clean reads to the reference mouse genome (GRCm38.p6)
Assembly: GRCm38.p6
Supplementary files format and content: comma-separated values (.csv) file including unnormalised gene count data for each sample
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| Submission date |
Jul 13, 2022 |
| Last update date |
Jul 17, 2024 |
| Contact name |
Robert J Williams |
| E-mail(s) |
rjw38@bath.ac.uk
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| Organization name |
University of Bath
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| Street address |
University Of Bath, The Avenue, Claverton Down
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| City |
Bath |
| ZIP/Postal code |
BA2 7AY |
| Country |
United Kingdom |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE208144 |
Epigallocatechin Gallate modulates microglia phenotype |
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| Relations |
| BioSample |
SAMN29719428 |
| SRA |
SRX16203544 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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