|
| Status |
Public on Jan 20, 2011 |
| Title |
hESCs-HLCs_P2_Passage 60_replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
H1
|
| Organism |
Homo sapiens |
| Characteristics |
strain: H1-derived hepatocyte-like cells (protocol 2)
developmental stage: Passage 60
cell type: hESCs-derived hepatocyte-like cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
| Label |
biotin
|
| Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using rank invariant normalisation with BeadStudio version 3 (Illumina)
|
| |
|
| Submission date |
Dec 01, 2010 |
| Last update date |
Jan 20, 2011 |
| Contact name |
Justyna Jozefczuk |
| E-mail(s) |
jagodzin@molgen.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Vertebrate Genomics
|
| Street address |
Ihnestrasse 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE25744 |
Comparative analysis of hESCs and iPSCs-derived hepatocyte-like cells reveals current drawbacks and possible strategies for improved differentiation |
|
