|
Status |
Public on Jan 20, 2011 |
Title |
hESCs-HLCs_P2_Passage 60_replicate 1 |
Sample type |
RNA |
|
|
Source name |
H1
|
Organism |
Homo sapiens |
Characteristics |
strain: H1-derived hepatocyte-like cells (protocol 2) developmental stage: Passage 60 cell type: hESCs-derived hepatocyte-like cells
|
Extracted molecule |
total RNA |
Extraction protocol |
The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
Label |
biotin
|
Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 1
|
Data processing |
The data were normalised using rank invariant normalisation with BeadStudio version 3 (Illumina)
|
|
|
Submission date |
Dec 01, 2010 |
Last update date |
Jan 20, 2011 |
Contact name |
Justyna Jozefczuk |
E-mail(s) |
jagodzin@molgen.mpg.de
|
Organization name |
Max Planck Institute for Molecular Genetics
|
Department |
Vertebrate Genomics
|
Street address |
Ihnestrasse 63
|
City |
Berlin |
ZIP/Postal code |
14195 |
Country |
Germany |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE25744 |
Comparative analysis of hESCs and iPSCs-derived hepatocyte-like cells reveals current drawbacks and possible strategies for improved differentiation |
|