Data table header descriptions |
ID_REF |
|
CH1D_MEDIAN |
Median spot pixel intensity at Channel 1 with the median-background subtracted (CH1I_MEDIAN - CH1B_MEDIAN) |
CH1I_MEDIAN |
Median spot pixel intensity at Channel 1 |
CH1_PER_SAT |
Percentage of spot pixels at Channel 1 that are saturated |
CH1I_SD |
Standard deviation of the spot intensity at Channel 1 |
CH1B_MEAN |
Mean spot background in Channel 1 |
CH1B_MEDIAN |
Median spot background intensity in Channel 1 |
CH1B_SD |
Standard deviation of the spot background intensity in Channel 1 |
CH1D_MEAN |
Mean Channel 1 intensity with the median-background subtracted (CH1I_MEAN - CH1B_MEDIAN) |
CH2I_MEAN |
Mean spot pixel intensity at Channel 2 |
CH2D_MEAN |
Mean spot pixel intensity at Channel 2 with the median-background subtracted (CH2I_MEAN - CH2B_MEDIAN) |
CH2D_MEDIAN |
Median spot pixel intensity at Channel 2 with the median-background subtracted (CH2I_MEDIAN - CH2B_MEDIAN) |
CH2I_MEDIAN |
Median spot pixel intensity at Channel 2 |
CH2_PER_SAT |
Percentage of spot pixels at Channel 2 that are saturated |
CH2I_SD |
Standard deviation of the spot pixel intensity at at Channel 2 |
CH2B_MEAN |
Mean spot background intensity in Channel 2 |
CH2B_MEDIAN |
Median spot background intensity in Channel 2 |
CH2B_SD |
Standard deviation of the spot background intensity in Channel 2 |
CH2BN_MEDIAN |
Normalized value of median Channel 2 background (CH2B_MEDIAN/Normalization factor) |
CH2DN_MEAN |
Normalized value of mean Channel 2 intensity with normalized background subtracted (CH2IN_MEAN - CH2BN) |
CH2IN_MEAN |
Normalized value of mean Channel 2 intensity (CH2I_MEAN/Normalization factor) |
CH2DN_MEDIAN |
Normalized value of median Channel 2 intensity with normalized background subtracted (CH2IN_MEDIAN - CH2BN) |
CH2IN_MEDIAN |
Normalized value of median Channel 2 intensity (CH2_MEDIAN/Normalization factor) |
CORR |
Coefficient of determination for the current regression value |
DIAMETER |
Diameter in µm of the spot indicator |
FLAG |
Type of flag associated with a spot (default=0) |
VALUE |
same as UNF_VALUE but with flagged values removed |
LOG_RAT2N_MEDIAN |
Log (base 2) of the ratio of the medians of Channel 2 to Channel 1 [log (base 2) (RAT2N_MEDIAN)] |
PIX_RAT2_MEAN |
Geometric mean of the pixel-by-pixel ratios of Channel 2 to Channel 1 pixel intensities, with the median background subtracted |
PIX_RAT2_MEDIAN |
Median of pixel by-pixel ratios of Channel 2 to Channel 1 pixel intensities, with the median background subtracted |
PERGTBCH1I_1SD |
Percentage of spot pixels with intensities more than one standard deviation above the background pixel intensity, at Channel 1 |
PERGTBCH1I_2SD |
|
PERGTBCH2I_1SD |
Percentage of spot pixels with intensities more than one standard deviation above the background pixel intensity, at Channel 2 |
PERGTBCH2I_2SD |
Percentage of spot pixels with intensities more than two standard deviations above the background pixel intensity, at Channel 1 |
RAT1_MEAN |
Ratio of the arithmetic mean intensities of each spot for each wavelength, with the median background subtracted. Channel 1/Channel 2 ratio, (CH1I_MEAN - CH1B_MEDIAN)/(CH2I_MEAN - CH2B_MEDIAN) or Green/Red ratio |
RAT1N_MEAN |
Ratio of the means of Channel 1 intensity to normalized Channel 2 intensity with median background subtracted (CH1D_MEAN/CH2DN_MEAN). Channel 1/Channel 2 ratio normalized or Green/Red ratio normalized |
RAT2_MEAN |
Ratio of the arithmetic mean intensities of each spot for each wavelength, with the median background subtracted. Channel 2/Channel 1 ratio, (CH2I_MEAN - CH2B_MEDIAN)/(CH1_MEAN - CH1B_MEDIAN) or Red/Green ratio |
RAT2_MEDIAN |
Ratio of the median intensities of each spot for each wavelength, with the median background subtracted |
RAT2_SD |
Standard deviation of pixel intensity ratios |
RAT2N_MEAN |
Channel 2/Channel 1 ratio normalized, RAT2N_MEAN/Normalization factor or Red/Green mean ratio normalized |
RAT2N_MEDIAN |
Channel 2/Channel 1 ratio normalized, RAT2N_MEDIAN/Normalization factor or Red/Green median ratio normalized |
REGR |
Regression ratio |
SUM_MEAN |
Sum of the arithmetic mean intensities for each wavelength, with the median background subtracted (CH1D_MEAN + CH2D_MEAN) |
SUM_MEDIAN |
Sum of the median intensities for each wavelength, with the median-background subtracted (CH1D_MEDIAN + CH2D_MEDIAN) |
TOT_BPIX |
Total number of background pixels |
TOT_SPIX |
Total number of signal (spot) pixels |
X_COORD |
X-coordinate in µm of the center of the spot-indicator associated with the spot, where (0,0) is the top left of the image |
Y_COORD |
Y-coordinate in µm of the center of the spot-indicator associated with the spot, where (0,0) is the top left of the image |
TOP |
Box top: int(((centerX - radius) - Xoffset) / pixelSize) |
BOT |
Box bottom: int(((centerX + radius) - Xoffset) / pixelSize) |
LEFT |
Box left: int(((centerY - radius) - yoffset) / pixelSize) |
RIGHT |
Box right: int(((centerY + radius) - yoffset) / pixelSize) |
UNF_VALUE |
aka LOG_RAT2N_MEAN, or Log (base 2) of the ratio of the mean of Channel 2 to Channel 1 [log (base 2) (RAT2N_MEAN)] |