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Sample GSM630447 Query DataSets for GSM630447
Status Public on Dec 09, 2010
Title wt-sv022-a
Sample type RNA
 
Channel 1
Source name refpool
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: refpool
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy5
Label protocol robot amplification and labeling v1.0
 
Channel 2
Source name wt
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: wt
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy3
Label protocol robot amplification and labeling v1.0
 
 
Hybridization protocol Tecan HS4800 hybridization
Scan protocol scanning of slides using Agilent G256BA
Features were extracted using Imagene software from Biodiscovery.
Data processing genes, no bg corr, dye corr: as genes, no bg corr, but followed by gene-specific dye bias correction. Identical to genes, no bg corr if the dye bias correction is 0.0, which is the default. So far (jan 2008), we have only dye-bias corrections for yeast.
limma: A software package for the analysis of gene expression microarray data, especially the use of linear models for analysing designed experiments and the assessment of differential expression. Author(s): Gordon Smyth. The limma R package version 2.12.0 is used. P-values are Benjamini-Hochberg FDR corrected.
 
Submission date Nov 29, 2010
Last update date Jun 06, 2013
Contact name Patrick Kemmeren
Organization name UMC Utrecht
Department Department of Molecular Cancer Research
Lab Holstege Lab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CG
Country Netherlands
 
Platform ID GPL11232
Series (4)
GSE25644 Expression profiling of yeast kinase and phosphatase knockouts
GSE33097 Deletion mutant analysis of established glucose signaling and metabolic pathway members in Saccharomyces cerevisiae
GSE33099 Glucose signaling

Data table header descriptions
ID_REF
VALUE normalized log2 ratio test/ref
Signal Norm_Cy5
Signal Norm_Cy3

Data table
ID_REF VALUE Signal Norm_Cy5 Signal Norm_Cy3
1 0.282613126815424 25658.5 31210.9
2 -0.059296644318287 355.393 341.082
3 0.195507288898808 835.766 957.058
4 -0.0409925220077112 46.8302 45.5183
5 -0.209956391339842 963.469 832.98
6 0.0367896821522282 194.397 199.418
7 -0.00899721055781888 845.265 840.01
8 0.0361530565351212 1751.26 1795.7
9 -0.118497717208035 605.717 557.954
10 -0.0670857944541817 59.7459 57.0313
11 -0.143851947454729 6516.92 5898.46
12 0.0129646063192835 49.6074 50.0552
13 0.0742758433935938 335.665 353.399
14 0.0214903872157823 245.344 249.026
15 -0.0616061112365078 5090.04 4877.26
16 -0.00405572182404241 73.6304 73.4237
17 0.0681676424485263 48.8733 51.238
18 -0.119628827244669 49.7238 45.767
19 -0.032530447680799 17994.2 17593
20 0.0669341860990078 1537.43 1610.44

Total number of rows: 15552

Table truncated, full table size 608 Kbytes.




Supplementary file Size Download File type/resource
GSM630447.txt.gz 657.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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