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Sample GSM630379 Query DataSets for GSM630379
Status Public on Dec 09, 2010
Title wt-ic007-a
Sample type RNA
 
Channel 1
Source name refpool
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: refpool
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy5
Label protocol robot amplification and labeling v1.0
 
Channel 2
Source name wt
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: wt
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy3
Label protocol robot amplification and labeling v1.0
 
 
Hybridization protocol Tecan HS4800 hybridization
Scan protocol scanning of slides using Agilent G256BA
Features were extracted using Imagene software from Biodiscovery.
Data processing genes, no bg corr, dye corr: as genes, no bg corr, but followed by gene-specific dye bias correction. Identical to genes, no bg corr if the dye bias correction is 0.0, which is the default. So far (jan 2008), we have only dye-bias corrections for yeast.
limma: A software package for the analysis of gene expression microarray data, especially the use of linear models for analysing designed experiments and the assessment of differential expression. Author(s): Gordon Smyth. The limma R package version 2.12.0 is used. P-values are Benjamini-Hochberg FDR corrected.
 
Submission date Nov 29, 2010
Last update date Jun 06, 2013
Contact name Patrick Kemmeren
Organization name UMC Utrecht
Department Department of Molecular Cancer Research
Lab Holstege Lab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CG
Country Netherlands
 
Platform ID GPL11232
Series (5)
GSE25644 Expression profiling of yeast kinase and phosphatase knockouts
GSE33097 Deletion mutant analysis of established glucose signaling and metabolic pathway members in Saccharomyces cerevisiae
GSE33099 Glucose signaling

Data table header descriptions
ID_REF
VALUE normalized log2 ratio test/ref
Signal Norm_Cy5
Signal Norm_Cy3

Data table
ID_REF VALUE Signal Norm_Cy5 Signal Norm_Cy3
1 -0.15467594270151 37283.9 33493.4
2 0.0162919677329958 181.126 183.183
3 0.0548528668013351 573.45 595.673
4 -0.246970584317824 57.7325 48.6491
5 -0.0283031718394949 365.006 357.915
6 -0.0485248648799871 173.633 167.89
7 -0.0359792096038449 416.354 406.099
8 -0.0583694856649129 555.722 533.687
9 0.0959466417757553 281.329 300.675
10 0.120854161214417 71.6664 77.9285
11 -0.0837367333143092 1926.42 1817.79
12 -0.244162590864263 61.0684 51.5604
13 -0.177112892373237 331.623 293.311
14 -0.127228661045746 117.567 107.643
15 -0.0965273945844127 1299.32 1215.23
16 -0.0396392609882179 68.3017 66.4506
17 -0.170766394119911 59.2156 52.6054
18 -0.100212956434163 25354.4 23653
19 0.194031588068033 8734.89 9992.33
20 -0.0545447276436278 338.286 325.735

Total number of rows: 15552

Table truncated, full table size 612 Kbytes.




Supplementary file Size Download File type/resource
GSM630379.txt.gz 651.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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