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Sample GSM630223 Query DataSets for GSM630223
Status Public on Dec 09, 2010
Title pkh1-del-1-a
Sample type RNA
 
Channel 1
Source name refpool
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: refpool
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy5
Label protocol robot amplification and labeling v1.0
 
Channel 2
Source name pkh1-del
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: pkh1-del
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy3
Label protocol robot amplification and labeling v1.0
 
 
Hybridization protocol Tecan HS4800 hybridization
Scan protocol scanning of slides using Agilent G256BA
Features were extracted using Imagene software from Biodiscovery.
Data processing genes, no bg corr, dye corr: as genes, no bg corr, but followed by gene-specific dye bias correction. Identical to genes, no bg corr if the dye bias correction is 0.0, which is the default. So far (jan 2008), we have only dye-bias corrections for yeast.
limma: A software package for the analysis of gene expression microarray data, especially the use of linear models for analysing designed experiments and the assessment of differential expression. Author(s): Gordon Smyth. The limma R package version 2.12.0 is used. P-values are Benjamini-Hochberg FDR corrected.
 
Submission date Nov 29, 2010
Last update date Jun 06, 2013
Contact name Patrick Kemmeren
Organization name UMC Utrecht
Department Department of Molecular Cancer Research
Lab Holstege Lab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CG
Country Netherlands
 
Platform ID GPL11232
Series (5)
GSE25644 Expression profiling of yeast kinase and phosphatase knockouts
GSE33097 Deletion mutant analysis of established glucose signaling and metabolic pathway members in Saccharomyces cerevisiae
GSE33099 Glucose signaling

Data table header descriptions
ID_REF
VALUE normalized log2 ratio test/ref
Signal Norm_Cy5
Signal Norm_Cy3

Data table
ID_REF VALUE Signal Norm_Cy5 Signal Norm_Cy3
1 -0.21707179842565 31431.7 27041
2 -0.0048950102670784 502.478 500.776
3 0.028185643236171 1299.11 1324.74
4 -0.293117605554425 56.053 45.7469
5 -0.0250800074778727 1199.38 1178.71
6 -0.0693727742087079 283.102 269.811
7 0.0389366080175652 1173.77 1205.88
8 -0.0427434320643843 2705.11 2626.14
9 0.0101586054107936 931.469 938.051
10 0.0437070304756798 65.4378 67.4506
11 -0.0318095949713194 10725.7 10491.8
12 -0.0940610767876127 59.5058 55.7499
13 -0.112811727249499 489.829 452.986
14 0.0174454612458357 392.662 397.439
15 -0.0848018815440612 8349.86 7873.2
16 0.0398661076629893 94.4022 97.0472
17 -0.142232098590877 75.9023 68.7763
18 -0.372234396732447 43391.6 33523.7
19 0.0958810467222045 27193.8 29062.5
20 -0.00465574927477432 1800.17 1794.37

Total number of rows: 15552

Table truncated, full table size 610 Kbytes.




Supplementary file Size Download File type/resource
GSM630223.txt.gz 669.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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